A Study to Investigate the Osteogenic Potential of Peptide AC - 100

Introduction: Titanium alloy is a commonly used implant material in orthopaedics but improving its interface with bone is a key goal in its application to new tissue engineering approaches. In this project we investigated the osteogenic potential of AC-100 (an active motif from the Matrix Extracellular Phosphoglycoprotein) and in doing so further studies could be carried out on titanium surfaces. Methods: AC-100 peptide was attached to glass cover slips to influence rat calvarial osteoblast adhesion and compared it to adsorbed fibronectin (FN) and plain glass (control surface). Results: The results were analysed with ANOVA. Glass surfaces coated with AC-100 and FN showed osteoblast adhesion and spreading significantly greater than the control surface (p < 0.05). Conclusion: We demonstrated that AC-100 when compared to Fibronectin provides a suitable environment for osteoblast adhesion on glass surface, which may translate to offering a suitable environment for osteoblast adhesion on titanium.